UNCERTAINTY ANALYSIS OF CELL COUNTING BY METABOLIC ASSAYS

• Abstract:

  Cell counting is an important procedure in cell biology and its uncertainty evaluation is fundamental to improve cell-based measurement comparability which is currently variable. In this study, for the first time, the uncertainty of a metabolic assay for cell counting based on fluorescence intensity measurement has been evaluated. Results, in 2D cell culture, revealed that three are the main components: repeatability and reproducibility of the measurement system and a contribution due to the sample loading step procedure. This study represents the basis of a 3D cell culture in which metabolic methodologies are the most suitable method to monitor cell growth.
• Keywords:

  2D and 3D cell culture, scaffold, cell proliferation rate, CellTiter Blue, resazurin, uncertainty analysis
• Download:

  IMEKO-WC-2012-SS2-O11.pdf
• DOI:

  _unreg_wc-2012.SS2-O11

Event details: