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Eventual limits of the current EU official method for evaluating milk adulteration of water buffalo dairy products and potential proteomic solutions

Caira, S., Nicolai, M. A., Lilla, S., Calabrese, M. G., Pinto, G., Scaloni, A., Chianese, L., Addeo, F.
  • Abstract:
    The European reference method (ERM) identifies the fraudulent addition of bovine (B) milk in water buffalo (WB) milk/dairy products by concomitant isoelectric focusing (IEF) detection of B γ2- and γ3-CN fragments after plasminolysis. False positive results could occurr as the WB β-CN (f100- 209), also formed after plasminolysis of WB milk/dairy products comigrates with B γ2-CN. These ERM limitations were overcome by a phosphopeptides enrichment procedure followed by trypsinolysis and the elution of B β-CN(f1-25)4P and WB β-CN(f1-28)4P peptides identified as suitable specie/specific markers with a detection limit of 0.8% v/v.
  • Keywords:
    gamma-2- and gamma-3-casein, peptide markers, milk, cheese, proteomics
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  • DOI:
    _unreg_tc23-2017.144

Event details:

  • IMEKO TC:
    TC23
  • Event name:
    3rd IMEKOFOODS Conference
  • Title:

    Metrology Promoting Standardization and Harmonization in Food and Nutrition

  • Place:
    Thessaloniki, GREECE
  • Time:
    1 October 2017 - 4 October 2017