Two-photon calcium imaging experiments to investigate the properties of neuronal microcircuits: the example of cerebellar network activity

• Abstract:

  We have developed a custom-made two-photon microscope integrating a spatial light modulator that can modulate the phase of a laser beam and divide it into multiple diffraction-limited beamlets configurable in real time. We used this system to study cerebellar circuit activity and long-term synaptic plasticity expression by simultaneously acquiring stimulus-induced calcium signals from neurons located in different cerebellar layers.
• Download:

  IMEKO-TC2-2025-018.pdf
• DOI:

  tc2-2025.018

Event details:

• IMEKO TC:

  TC2
• Event name:

  IMEKO TC2 PhotoMet 2025
• Title:

  2025 IMEKO TC2 International Symposium on Modern Photonic Metrology

  PhotoMet 2025 - Shaping the Future of Photonic Metrology

• Place:

  Modena, ITALY
• Time:

  1 September 2025 - 3 September 2025