Disposable electrochemical biosensors based on screen-printing technology for malic acid determination in wine

Larraitz Anorga, Arrate Jaureguibeitia, Graciela Martínez-Paredes, Pedro J. Lamas-Ardisana, Elena Jubete, Oscar A. Loaiza, Germán Cabanero, Asier Albizu, Hans J. Grande

Abstract

This work describes the fabrication of a disposable electrochemical biosensor based on screen-printing technology for malic acid detection in wine. The new biosensor was constructed using ferricyanide as electrochemical mediator and the Malate Quinone Oxidoreductase (MQO) enzyme. It showed great analytical performance in terms of reproducibility (RSD 4%; <i>n</i> = 5), sensitivity (37 µA/g/l), detection limit (0,33 g/l) and stability on time (1 month at RT). Moreover, the disposable biosensor was successfully applied for the determination of malic acid in wines.

Keywords

screen-printing, biosensor, malic acid, Malate Quinone Oxidoreductase, wine

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DOI

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IMEKO TC

TC23 - Metrology in Food and Nutrition

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